DOS-based Windows OS's include Windows 95, Windows 98 and Windows Millenium.
Rename to .isoDOS01 - CD files will be on B: drive. 
It is best to remove some unneeded files from the Win98 ISO before using it.
See RMPrepUSB Tuto rial 117.
See blog post here
You can also make a .imgPTN file.


Win95/98/Me are very old. They will therefore only run on very old systems with small IDE hard drives (or BIOS configured for Legacy IDE) and less than 1GB of RAM. DOS can get very confused about large hard disks and systems with large RAM memory!
Even if you manage to get it installed, you will probably not be able to run a browser or get audio working. Graphics will also be limited. No USB access, WiFi, Bluetooth, Enet, etc.

Recommended for Easy2Boot (fastest flash drive!)
SanDisk Extreme SDCZ880-128G-G46

Fastest USB 3!
See Blog post